FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18636797
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC428300.010.855682120
CGTCTGA330950.010.6771755120
ACGTCTG365700.09.744621120
CACGTCT404350.09.555027120
GCACACG540300.09.227192120
CACACGT494250.08.994458120
TCGGAAG975100.08.45974120
ATCGGAA1019850.08.4003105120
CGGAAGA924700.08.31097120
AGCACAC661500.08.26213120
GAGCACA727800.08.210144120
AGAGCAC762850.08.202545120
AGTCACG121300.08.061768120
GATCGGA1080200.08.04207120
AGATCGG1165550.07.9524517120
TCTGAAC387050.07.9360857120
GTCTGAA416650.07.804254120
TAGATCG348800.07.5507746120
AAGATCG378800.07.3645544120
AAGAGCA906100.07.3559947120
Produced by FastQC (version 0.11.5)