FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18636797
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT402700.010.908331120
TCGTGTA363850.010.42374120
CGTGTAG340100.010.339982120
AGCGTCG521700.010.134069120
CGTCGTG434600.010.0248120
GCGTCGT469300.09.743911120
GAGCGTC586750.09.624216120
GTGTAGG361700.09.490222120
AGAGCGT636650.09.15266120
GTAGGGA326600.09.132066120
AAGAGCG712800.09.008343120
TCGGAAG956350.08.452391120
ATCGGAA1003950.08.386378120
TGTAGGG369750.08.358491120
CGGAAGA911650.08.195398120
GATCGGA1063400.08.075544120
GCGTCGG37400.08.022823120
AGATCGG1144800.07.941671120
TAGGGAA329250.07.3635025120
AAGATCG375600.07.3176017120
Produced by FastQC (version 0.11.5)