FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48829812
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG687050.011.963583120
AGCGTCG846800.011.855001120
GTCGTGT629100.011.605398120
GCGTCGT749100.011.293208120
TCGTGTA556100.011.21783120
CGTGTAG527700.011.184398120
GAGCGTC988800.011.087624120
AGAGCGT1078250.010.824876120
AAGAGCG1246800.010.454643120
TCGGAAG1779450.010.237077120
ATCGGAA1875200.010.217047120
GATCGGA2015200.010.204426120
AGATCGG2224250.010.092948120
CGGAAGA1692700.09.7969265120
GTGTAGG600300.09.411686120
CAGATCG1142950.09.250778120
TAGATCG282450.08.269023120
GAAGAGC1813050.08.163067120
TGTAGGG648800.07.884514120
GAGATCG808350.07.642969120
Produced by FastQC (version 0.11.5)