FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17656822
Sequences flagged as poor quality0
Sequence length126
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC26900.018.288648
TTCCGAT32450.015.3474887
CCGATCT43900.011.4798069
ATCGGAA526900.011.363763120
ACACGTC186200.011.051831120
GATCGGA578100.010.89698120
AGATCGG644300.010.559534120
CGGAAGA495350.010.537241120
CGTCTGA144700.010.448448120
TCGGAAG513250.010.134678120
ACGTCTG159900.010.093078120
CACGTCT184150.010.067151120
CACACGT242750.09.93543120
CAGATCG403450.09.591572120
ACGCTCT52550.09.3726091
AGAGCAC420350.09.305856120
GCACACG284550.08.391591120
AAGAGCA512600.08.380197120
CTTCCGA66200.08.2501046
GAGCACA409000.07.9798617120
Produced by FastQC (version 0.11.5)