FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19136200
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA235650.011.533416120
ACACGTC308900.010.973816120
CACGTCT294200.010.563655120
GATCGGA918550.010.267788120
ACGTCTG262500.010.148008120
TCGGAAG810400.010.112964120
CACACGT373450.010.0570135120
CGGAAGA759700.09.895462120
AGATCGG1011150.09.796222120
ATCGGAA850150.09.760089120
TAGATCG178700.09.702875120
GCACACG408800.09.480883120
ACGTCCG45900.09.280531120
AGCACAC522550.09.081881120
AGAGCAC616500.08.924079120
GAGCACA591250.08.848557120
GAGATCG327750.08.43889120
CAGATCG423700.08.354505120
GAAGAGC796100.08.25981120
AAGAGCA757500.08.157963120
Produced by FastQC (version 0.11.5)