FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201278
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAA3154.364123E-617.179224120
ACACGTC58950.012.443644120
GTCACGT10800.012.248149120
CACGTCT53950.011.925152120
CACACGT67450.011.49951120
CGTCTGA43650.010.882142120
CGTTTCG5855.456968E-1210.772751118-119
ACGTCTG48300.010.456918120
TTCGGAA6905.427619E-610.456918120
GCACACG73900.010.2739935118-119
AAGAGCA117650.010.016954120
TCCAGTC26550.09.738128120
TCTGGCG2809.719897E-49.64259690-91
TCTGAAC45000.09.620364120
GAGCACA95250.09.595115120
AGAGCAC100900.09.594145120
CGGAGAT4451.1523553E-69.441288118-119
AGCACAC83550.09.283565120
TCACGTT10400.09.25035120
TGAACTC39300.09.179737120
Produced by FastQC (version 0.11.5)