FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96169121
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2231650.010.380626120
CACACGT2569950.09.807455120
GCACACG2838900.09.778653120
ACGTCTG1909550.09.6876545120
GATCGGA6197700.09.57454120
CGTCTGA1728750.09.50064120
CACGTCT2115600.09.48322120
ATCGGAA5753400.09.439012120
TCGGAAG5440100.09.351376120
CGGAAGA5125350.09.236872120
AGATCGG6744350.09.076708120
AGCACAC3474800.08.683157120
CAGATCG3882550.08.6665535120
AGAGCAC4083950.08.6205635120
GAAGAGC5135350.08.408484120
GAGCACA3877250.08.372832120
AAGAGCA4863800.07.9048266120
TAGATCG495150.07.7976117120
GAGATCG2319550.07.7600827120
GTCACGA618750.07.348013120
Produced by FastQC (version 0.11.5)