FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96169121
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1371650.1426289421944493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG206750.014.533161120
CGTCGTG2288300.010.644141120
AGCGTCG2768950.010.492701120
GTCGTGT2112350.010.202366120
TCGTGTA1904750.010.160451120
CGTGTAG1793550.09.93764120
GAGCGTC3135450.09.869241120
AAGAGCG3821850.09.700644120
GCGTCGT2463300.09.677724120
GATCGGA6088750.09.492988120
ATCGGAA5660400.09.38799120
AGAGCGT3393300.09.381928120
TCGGAAG5359350.09.335588120
AGATCGG6631900.09.063265120
CGGAAGA5063250.09.057381120
GTGTAGG1887500.08.581641120
CAGATCG3796600.08.383732120
GAAGAGC5099350.08.249332120
GAGATCG2353100.08.068422120
TGTAGGG1939850.07.974455120
Produced by FastQC (version 0.11.5)