FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13449816
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG35350.020.095867120
TCGTGTA502750.09.208489120
CGTCGTG591350.09.050473120
GTGTAGG472750.08.977837120
GAGCGTC759450.08.735677120
CGTGTAG473150.08.601258120
GTCGTGT546800.08.598777120
AGCGTCG688200.08.389153120
TGTAGGG471050.08.294531120
GTAGGGA418100.08.135466120
GCGTCGT629500.08.115195118-119
TAGGGAA402100.08.099856120
AGAGCGT811000.07.9280167120
GAGTGTA216150.07.826457120
AAGAGCG897700.07.786013120
AGTGTAG206450.07.727608120
CGGAAGA1102800.07.489822120
GATCGGA1275300.07.3122864120
ATCGGAA1203650.07.2474008120
AGAGCGG62500.07.1279693120
Produced by FastQC (version 0.11.5)