FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49762579
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG93550.013.607792120
CGTGTAG791900.012.05653120
TCGTGTA846850.011.699654120
CGTCGTG1033100.011.496866120
GTCGTGT950250.011.279656120
AGCGTCG1257650.010.862185120
GAGCGTC1435350.010.354115120
GCGTCGT1117050.010.30493120
GTGTAGG830050.010.012152120
AGAGCGT1568250.09.897849120
AAGAGCG1791650.09.488166120
TCGGAAG2451050.09.275215120
ATCGGAA2573800.09.234142120
GTAGGGA752600.09.167509120
CGGAAGA2320050.09.01212120
GATCGGA2758050.08.969963120
AGATCGG2993800.08.602582120
TGTAGGG871400.08.56543120
TAGGGAA752900.08.43011120
TAGATCG843800.08.411501120
Produced by FastQC (version 0.11.5)