FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16892664
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG208350.08.871631120
CGTCGTG179400.08.864815120
GTCGTGT164100.08.630772120
GATCGGA389400.08.461018120
ATCGGAA367900.08.351921120
GCGTCGT186200.08.300807118-119
AGATCGG428350.08.294098120
CGTGTAG148350.08.293016120
TAGATCG130350.07.918878120
AGAGCGT253200.07.916423120
TCGGAAG367950.07.9104123120
TCGTGTA148100.07.741071118-119
GAGCGTC243000.07.730085120
AAGAGCG297100.07.6354623120
CGGAAGA367300.07.1564784120
CCCCGCG152500.06.6112814120
CGCGCGG90700.06.515085786-87
GTGTAGG181050.06.065949120
CCGCGCG96250.06.0454684-85
AAGATCG161850.05.7843976120
Produced by FastQC (version 0.11.5)