FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26674967
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC255500.012.105965120
GCACACG343950.010.861209120
GATCGGA775450.010.843196120
ACGTCTG219300.010.708318120
CGTCTGA192100.010.692564120
TCGGAAG685200.010.509569120
ATCGGAA721200.010.48463120
CACGTCT247950.010.391453120
AGATCGG857350.010.157643120
CGGAAGA649900.09.906754120
CACACGT320650.09.496418120
TAGATCG285000.09.145932118-119
AGAGCAC566150.08.592837120
AAGATCG285750.08.281197120
GAGCACA538000.08.17169120
CAGATCG196650.08.124016120
GAAGAGC730100.07.913631120
AGCACAC496650.07.8120527120
AAGAGCA746950.07.4938927120
GAGATCG353300.07.30984120
Produced by FastQC (version 0.11.5)