FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77030820
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA191400.011.411781120
ACGTCTG4720400.07.859836120
CGTCTGA4339800.07.790729120
CACGTCT5047100.07.541818120
ACACGTC5305150.07.5163684120
TCTGAAC4213700.07.41698120
CATCACG1430650.07.2425003120
GTCTGAA4508150.07.142091120
CACACGT5789800.07.141811120
GCACACG6182600.07.0520587120
CTGAACT4108600.06.993077120
CCAGTCA2594250.06.972137120
CTCGTAT693200.06.857365120
AACTCCA3555050.06.809153120
ATCACGA1412450.06.7607145120
GAGCACA7274850.06.7252536120
TCCAGTC2900300.06.719807120
AGCACAC6879650.06.7118807120
GTCACAT2099450.06.6578255120
TGAACTC4189250.06.5841103120
Produced by FastQC (version 0.11.5)