FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77030820
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG170600.028.791784120
AGAGCGG322150.08.587061120
CGTGTAG4541050.07.9804044120
TCGTGTA4779700.07.675252120
GTAGGGA3958450.07.6491723120
GTGTAGG4434250.07.5242968120
CGTCGTG5459100.07.4718695120
TAGGGAA3811550.07.4111104120
TGTAGGG4318100.07.3805504120
GTCGTGT5126050.07.37182120
GAGTGTA2204150.07.2924232120
AGCGTCG6234350.07.272602120
AGTGTAG2086650.07.2178297120
GAGCGTC6703800.07.12203120
GTAGATC2144850.06.974207120
GTCGTGG220600.06.966661120
GTGTAGA2036500.06.942783120
GCGTCGT5764950.06.934833118-119
TGGTCGC739400.06.8712873120
AAGAGCG7771400.06.8276424120
Produced by FastQC (version 0.11.5)