FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99591923
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1184100.010.7566805120
ATCGGAA3200800.010.1445265120
GATCGGA3435850.010.108322120
TCGGAAG3051550.09.986728120
AGATCGG3785100.09.864834120
CGGAAGA2891250.09.823175120
ACGTCTG1014200.09.790891120
CGTCTGA930000.09.707843120
GCACACG1580500.09.568677120
CACGTCT1146950.09.522395120
CACACGT1433150.09.399093120
CAGATCG1543350.08.747442120
TAGATCG641550.08.685303120
GAGATCG1377500.08.3606415120
AAGATCG1132350.08.31279120
AGAGCAC2465000.08.256685120
AGCACAC2137250.08.018213120
GAGCACA2376750.07.7969685120
GAAGAGC3215900.07.7044415120
AAGAGCA3138400.07.419712120
Produced by FastQC (version 0.11.5)