FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105809084
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3305650.09.446422120
CACGTCT3139550.09.251335120
CGTCTGA2592450.09.189213120
ACGTCTG2851350.09.1852665120
GCACACG4183750.08.791051120
CACACGT3777850.08.736568120
TCGGAAG7407550.08.54378120
GATCGGA8319600.08.507213120
CGGAAGA7012500.08.498038120
ATCGGAA7765700.08.446916120
AGATCGG8992950.08.188998120
AGAGCAC5655550.08.157776120
AGCACAC4899150.08.096144120
GAGCACA5374200.08.0011215120
TAGATCG1889350.07.7580824120
GAAGAGC6936100.07.6732135120
AAGAGCA6486200.07.6541567120
GTCTGAA2958450.07.5668845120
GAGATCG3468500.07.486824120
TCTGAAC2779200.07.43648120
Produced by FastQC (version 0.11.5)