FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105809084
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG322050.013.868313120
TCGTGTA2867250.09.799599120
CGTGTAG2721000.09.574183120
AGCGTCG4098200.09.499917120
CGTCGTG3443300.09.364613120
GTCGTGT3171250.09.346104120
GAGCGTC4575950.09.138165120
AAGAGCG5477550.08.865894120
GCGTCGT3680650.08.693675120
GTGTAGG2768450.08.616489120
AGAGCGT4896600.08.563116120
TCGGAAG7334600.08.518516120
GATCGGA8219000.08.465338120
ATCGGAA7682350.08.404797120
CGGAAGA6988800.08.379316120
GTAGGGA2475200.08.158781120
AGATCGG8903900.08.142044120
TAGATCG1847950.08.009609120
TGTAGGG2791800.07.8932967120
TAGGGAA2408250.07.590777120
Produced by FastQC (version 0.11.5)