FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16012570
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG52950.011.141379120
AGCGTCG559550.09.015378120
TCGTGTA398600.08.7744055120
CGTGTAG383700.08.691542120
GTCGTGT440000.08.673921120
CGTCGTG476400.08.668246120
GAGCGTC623050.08.183505120
AAGAGCG734600.07.9897385120
GCGTCGT505150.07.968754118-119
ATCGGAA965150.07.952327120
AGAGCGT662250.07.7769017118-119
GTGTAGG396450.07.7439156120
GTAGGGA353800.07.5884986120
TCGGAAG931850.07.5582056120
TAGGGAA338750.07.552461120
TGTAGGG405650.07.508927120
GATCGGA1025250.07.286531120
GAGTGTA190150.07.18635120
AGATCGG1099550.06.9400153118-119
TAGATCG318200.06.886207120
Produced by FastQC (version 0.11.5)