FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61307013
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG757400.011.677275120
CGTCGTG620350.011.607518120
GAGCGTC915500.010.8839035120
TCGTGTA520200.010.72888120
GCGTCGT661600.010.6654120
ATCGGAA1712300.010.544879120
AAGAGCG1153650.010.453263120
AGAGCGT993000.010.452808120
GTCGTGT585400.010.295001120
CGTGTAG495800.010.285416120
AGATCGG2026300.010.268688120
GATCGGA1828950.010.142263120
TCGGAAG1648750.010.0639715120
CGGAAGA1590000.09.458894120
TAGATCG355400.08.876849120
CAGATCG763300.08.842098120
GAGATCG830750.08.501052120
AAGATCG647100.08.392283120
GCGTCGG107550.07.8372307120
GTGTAGG619000.07.4115553120
Produced by FastQC (version 0.11.5)