FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49018198
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC629700.011.146806120
CGTCTGA482450.010.693358120
CACGTCT611600.010.689267120
GCACACG835600.010.431716120
ACGTCTG541600.010.403579120
GATCGGA1814850.010.196443120
ATCGGAA1690350.09.886177120
CGGAAGA1525800.09.800304120
CACACGT766700.09.783138120
TCGGAAG1612700.09.697754120
AGATCGG1990450.09.605385120
TAGATCG650250.08.813373120
AGAGCAC1299450.08.38503120
AGCACAC1125850.08.293098120
GAGCACA1241650.08.106299120
CAGATCG502500.07.810829120
AAGATCG684250.07.7596120
AAGAGCA1637950.07.758418120
GAAGAGC1674550.07.714667120
GAGATCG666350.07.3898644120
Produced by FastQC (version 0.11.5)