FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54882280
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG375500.011.469749120
AGCGTCG459100.011.409165120
GCGTCGT400000.010.647091120
GTCGTGT356150.010.507509120
ATCGGAA1012000.010.227028120
TCGTGTA316850.010.161452120
AAGAGCG716900.010.155206120
GATCGGA1076700.010.131315120
GAGCGTC563150.09.951814120
CGTGTAG312000.09.799589120
AGATCGG1188750.09.641232120
TCGGAAG995600.09.418087120
AGAGCGT603650.09.174669120
CGGAAGA984400.09.0858965120
TAGATCG387550.08.881199120
AAGATCG431750.08.069383120
CAGATCG335100.07.7975745120
GTGTAGG426100.07.1613827120
GAGATCG537950.06.7220116120
GAAGAGC1258950.06.135885120
Produced by FastQC (version 0.11.5)