FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53909453
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC679700.012.657532120
ACGTCTG565400.012.582955120
CGTCTGA497450.012.551742120
CACACGT826800.011.6254835120
CACGTCT642100.011.500654120
GCACACG918750.010.984757120
ATCGGAA1957300.09.971941120
GATCGGA2096900.09.943681120
CGGAAGA1780700.09.871892120
TCGGAAG1868700.09.513032120
AGATCGG2293500.09.222189120
AGCACAC1245200.09.218683120
AGAGCAC1484200.09.048856120
TAGATCG518050.08.877225120
GAGCACA1418050.08.649618120
AAGATCG827750.08.64564120
GTCACGC137100.08.407829120
GAAGAGC1931750.08.322997120
AGTCACG158500.08.105964120
CAGATCG674700.08.061904120
Produced by FastQC (version 0.11.5)