FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49694057
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG644650.012.323989120
TCGTGTA529450.011.979464120
GTCGTGT598350.011.873634120
AGCGTCG797500.011.730128120
CGTGTAG497650.011.62359120
GAGCGTC933000.011.312835120
GCGTCGT700700.011.115522120
AGAGCGT1020900.010.897172120
AAGAGCG1177900.010.7284565120
ATCGGAA1786350.010.157867120
AGATCGG2098450.09.959605120
GCGTCGG79650.09.944328120
TCGGAAG1695200.09.925316120
GATCGGA1906050.09.809581120
CGGAAGA1604800.09.65808120
GTGTAGG581500.09.132315120
AAGATCG665700.08.608195120
TAGATCG475750.08.46313120
GAGATCG769550.08.148294120
GTAGGGA543650.07.935911120
Produced by FastQC (version 0.11.5)