FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911956
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA58450.09.4411545120
TAGATCG60200.09.266341120
ACACGTC72450.08.858644120
GCACACG94450.08.19237120
GATCGGA165700.08.1810255120
ATCGGAA157700.08.139616120
CACGTCT73250.08.106798120
CGGAAGA147450.08.095247120
ACGTCTG64600.07.985236120
AGATCGG181450.07.9667625120
TCGGAAG153700.07.922167120
CACACGT88550.07.654409120
AGAGCAC135500.07.038493120
GAAGAGC173050.06.7243752120
CAGATCG47350.06.713944120
AAGATCG60500.06.395383118-119
AGCACAC121350.06.326915120
GAGCACA132300.06.098523118-119
TCTGAAC84750.05.8743553120
AAGAGCA171850.05.8638325120
Produced by FastQC (version 0.11.5)