FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68550384
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG113550.016.92514120
AGCGTCG1709350.011.014785120
CGTCGTG1409150.010.872332120
CGTGTAG1109150.010.68332120
GAGCGTC1946200.010.529094120
TCGTGTA1189100.010.465038120
GTCGTGT1308300.010.337856120
GCGTCGT1516900.09.850604120
AAGAGCG2392500.09.734761120
ATCGGAA3562400.09.461162120
AGAGCGT2125450.09.451803120
GATCGGA3807250.09.3606415120
TCGGAAG3368200.09.3308735120
CGGAAGA3172400.09.134375120
AGATCGG4144650.09.104344120
GTGTAGG1185900.09.060074120
CAGATCG2119500.08.514918120
TAGATCG363100.08.319768120
GTAGGGA1075300.08.076219120
GAAGAGC3241650.08.049949120
Produced by FastQC (version 0.11.5)