FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48323179
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG674500.011.638962120
TCGTGTA561100.011.625461120
AGCGTCG820750.011.577538120
GTCGTGT626900.011.507086120
CGTGTAG520100.011.225354120
GCGTCGG79750.010.996203120
GAGCGTC961500.010.788565120
GCGTCGT722950.010.533289118-119
AAGAGCG1199450.010.005388120
AGAGCGT1042450.09.93927120
ATCGGAA1737400.09.486476120
GTGTAGG593750.09.387825120
GATCGGA1845150.09.013882120
AGATCGG2011050.08.870624120
TCGGAAG1659000.08.79793120
CGGAAGA1589700.08.626038120
TAGATCG625200.08.406393120
GTAGGGA558300.08.1872425120
CAGATCG524550.08.026971120
TGTAGGG660150.07.5973907120
Produced by FastQC (version 0.11.5)