FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59652394
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG815100.010.484188120
GTCGTGT643800.09.946016120
CGTCGTG680550.09.841015120
GAGCGTC957950.09.772766120
GCGTCGT725250.09.6068325120
ATCGGAA1680550.09.563015120
AAGAGCG1172900.09.557661120
TCGTGTA577850.09.51297120
AGATCGG1957750.09.352349120
GATCGGA1778300.09.202712120
CGTGTAG561350.09.193915120
TCGGAAG1623800.09.0915575120
AGAGCGT1026200.08.959061120
CGGAAGA1575100.08.896403120
CAGATCG670600.08.259883120
TAGATCG403900.08.00849120
GTGTAGG662650.07.8518257120
AAGATCG582450.07.6347585120
GCGTCGG107700.07.4666333120
GAGATCG883900.07.291836120
Produced by FastQC (version 0.11.5)