FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10697277
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG99050.09.330546120
AGCGTCG136050.09.263212120
CGTCGTG115900.08.750726120
AAGAGCG199000.08.594738120
GCGTCGT122650.08.562712120
TCGTGTA98850.08.560067118-119
GAGCGTC159000.08.492318120
AGAGCGT171100.08.312643120
TCGGAAG257000.08.149541120
ATCGGAA258050.08.093123120
GTCGTGT111900.08.044555120
GATCGGA271250.08.03115120
AGATCGG295050.07.952835120
CGGAAGA253150.07.514882120
TAGATCG83600.07.250299120
AAGATCG106700.06.496094118-119
GTGTAGG118650.06.2718377120
GCGTCGG23657.3435876E-75.5825467120
GAAGAGC305500.05.578892120
GAGATCG137850.05.5724225120
Produced by FastQC (version 0.11.5)