FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16459594
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC30500.027.145198
ACGCTCT34700.024.079461
TTCCGAT38550.021.4783027
CTTCCGA41650.020.0297576
CGCTCTT51700.016.394142
TCTTCCG57650.014.7830815
CGATCTA12100.08.67693410-11
CAGATCG154450.08.429387120
TCCCGAA48350.06.824807120
CGTATGC188750.05.975786120
TCGTATG205300.05.7570686120
GCTCTTC165550.05.55511763
CTCGTAT196800.05.5330734118-119
ACACGTC773200.05.501467120
GCACACG875100.05.40933120
TATGCCG169900.05.4028416120
CCGTCTT171250.05.3952837120
ATCGGAA1324400.05.313773120
CGTCTGA690800.05.2978745120
CACGTCT750300.05.2775598120
Produced by FastQC (version 0.11.5)