FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64080081
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2028090.3164930456314498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG104200.017.982658120
CGTCGTG1545350.010.625239120
CGTGTAG1215000.010.261672120
AGCGTCG1867350.010.230692120
TCGTGTA1306900.010.183439120
GAGCGTC2108750.010.076269120
GTCGTGT1431600.09.955042120
AAGAGCG2570500.09.696111120
GCGTCGT1670500.09.671033120
GTGTAGG1272350.09.345997120
AGAGCGT2287950.09.318562120
TCGGAAG3556550.09.052821120
ATCGGAA3772700.08.978295120
GATCGGA4008350.08.777094120
CGGAAGA3357150.08.660269120
AGATCGG4339150.08.5356455120
TGTAGGG1302350.08.309868120
GTAGGGA1148600.08.083654120
GAAGAGC3371450.07.9056506120
CAGATCG2053500.07.782465120
Produced by FastQC (version 0.11.5)