FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182732610
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2538650.09.193336120
GATCGGA6322800.09.041655120
ATCGGAA5948600.08.913474120
AGATCGG6902400.08.757347120
TCGGAAG5728900.08.72306120
ACGTCTG2230700.08.549899120
CACGTCT2484450.08.435829120
TAGATCG1012450.08.4278755120
CGTCTGA2031700.08.417464120
CGGAAGA5508950.08.340642120
GCACACG3294600.08.327668120
CACACGT3045050.08.315533120
CAGATCG3176250.07.645728120
AAGATCG1882200.07.562103120
AGAGCAC4898200.07.3900876120
AGCACAC4330600.07.2788897120
GAGCACA4761050.07.1599813120
GAGATCG2473850.07.139551120
GAAGAGC6234100.06.7431245120
AAGAGCA6116600.06.495333120
Produced by FastQC (version 0.11.5)