FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182732610
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5462080.298911070114962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2995600.09.971657120
CGTCGTG2507450.09.7574120
GCGTCGG347900.09.61493120
GAGCGTC3453800.09.335586120
TCGTGTA2127050.09.308647120
GTCGTGT2352650.09.209892120
CGTGTAG2048050.08.984493120
GATCGGA6247250.08.968878120
AAGAGCG4213400.08.935337120
ATCGGAA5882050.08.860052120
GCGTCGT2667200.08.780226118-119
AGATCGG6821250.08.768811120
TCGGAAG5688600.08.746464120
AGAGCGT3690600.08.689396120
TAGATCG994650.08.66417120
CGGAAGA5511400.08.201727120
CAGATCG3104950.07.8120427120
GTGTAGG2382100.07.1220303120
AAGATCG1900100.07.051288120
GAGATCG2551150.06.8078136120
Produced by FastQC (version 0.11.5)