FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68501887
Sequences flagged as poor quality0
Sequence length126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1850150.012.087198120
CGTCTGA1662000.012.026422120
ACACGTC2251000.012.010392120
CACGTCT2075250.011.662773120
GCACACG2815650.011.269763120
CACACGT2579200.011.195501120
AGCACAC3409700.010.032893120
GTCTGAA1964000.09.849541120
GAGCACA3788750.09.794132120
AGAGCAC4070950.09.611505120
TCTGAAC1810400.09.436329120
CGGAAGA5337950.09.35785120
GAACTCC1362600.09.183539120
TCGGAAG5701800.09.068645120
TGAACTC1578150.09.034199120
ATCGGAA6092550.08.926225120
CTGAACT1778250.08.917101120
AAGAGCA4812500.08.856443120
GAAGAGC5047000.08.851226120
GATCGGA6521950.08.6713705120
Produced by FastQC (version 0.11.5)