FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68501887
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG101400.019.926363120
CGTGTAG1773450.012.363017120
GTCGTGT2175550.012.095811120
TCGTGTA1949250.011.923636120
CGTCGTG2379600.011.831892120
AGCGTCG2926100.011.500463120
GTGTAGG1763500.011.259739120
GCGTCGT2611050.011.193034120
TGTAGGG1714750.011.113432120
GAGCGTC3291750.010.944583120
GTAGGGA1503650.010.714026120
AAGAGCG4047900.010.361942120
AGAGCGT3607600.010.316416120
TAGGGAA1521000.010.097607120
CGGAAGA5300250.09.320474120
TCGGAAG5656000.09.113801120
ATCGGAA6027900.08.927609120
GAAGAGC5024150.08.835644120
GATCGGA6449450.08.601426120
AGATCGG6942650.08.305673120
Produced by FastQC (version 0.11.5)