FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18268384
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG37450.023.284718120
CGTGTAG717200.011.638686120
TCGTGTA781300.011.299599120
CGTCGTG943500.011.039788120
GAGTGTA255950.010.808301120
GTAGGGA592200.010.78477120
GTGTAGG694150.010.7169485120
GTCGTGT862950.010.655568120
TAGGGAA566950.010.331633120
TGTAGGG665800.010.143568120
GCGTCGT1026450.010.094905120
AGCGTCG1138050.010.082582120
GAGCGTC1263500.010.066764120
AGTGTAG241300.09.296217120
AGAGCGT1370800.09.186654120
AAGAGCG1522700.09.0798645120
TGGTCGC55850.08.82969120
GTAGATC282850.08.717309120
GATCTCG134350.08.236352120
CGGAAGA1918300.08.194903120
Produced by FastQC (version 0.11.5)