FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140298881
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3584250.010.0972595120
CGTCTGA2762900.09.784971120
ACGTCTG3058900.09.7105120
CACGTCT3385600.09.652415120
GCACACG4471650.09.551032120
CACACGT4117700.09.488425120
TCGGAAG8463050.09.083008120
ATCGGAA8940500.09.023094120
GATCGGA9575800.09.014528120
CGGAAGA7943450.09.00147120
AGCACAC5493700.08.728106120
AGATCGG10388950.08.706138120
AGAGCAC6385600.08.582544120
GAGCACA6072350.08.470755120
TAGATCG1569850.08.207756120
GAAGAGC7927600.07.998223120
CAGATCG4275100.07.945618120
AAGAGCA7571200.07.8615475120
GAGATCG3438150.07.8176575120
AAGATCG2770250.07.7063127120
Produced by FastQC (version 0.11.5)