FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140298881
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4544280.3238999461442604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG282350.015.998821120
AGCGTCG4420200.010.439756120
CGTCGTG3652550.010.374172120
TCGTGTA3062250.010.106322120
CGTGTAG2882900.010.024524120
GTCGTGT3387700.010.014925120
GAGCGTC4969100.09.946596120
AAGAGCG6041500.09.527289120
GCGTCGT3939400.09.36412120
AGAGCGT5385150.09.252871120
TCGGAAG8368200.09.044747120
ATCGGAA8814500.09.028396120
GATCGGA9442600.08.950134120
CGGAAGA7890800.08.861146120
GTGTAGG3022750.08.809544120
AGATCGG10250050.08.702202120
TAGATCG1506150.08.495116120
GTAGGGA2718000.08.210457120
TGTAGGG3086250.08.139746120
CAGATCG4209450.08.017045120
Produced by FastQC (version 0.11.5)