FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618524
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT167050.010.385955120
ACCGTAC206600.09.476621120
GTACGTA166500.09.443363120
CACCGTA234400.09.252207120
CCGTACG180550.09.108892120
CGTCTGA817900.09.044792120
ACGTCTG893000.08.857552120
CACGTCT966550.08.713312120
GTCACCG310250.08.330017120
GCACACG1204850.08.329951120
TACGTAA155750.08.31593120
CGTAATC119600.08.310998120
ACACGTC1017100.08.167724120
TCTGAAC807750.08.09195120
CACACGT1124800.08.012291120
GTCTGAA869100.07.9920826120
CTGAACT790800.07.8844995120
TCACCGT310950.07.826926120
TCCAGTC534650.07.7070284120
AGTCACC419200.07.472785120
Produced by FastQC (version 0.11.5)