FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618524
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN599320.32189447455662973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG45500.021.520645120
CGTGTAG850600.09.294148120
TCGTGTA902650.08.904629120
GAGTGTA365750.08.836433120
GTGTAGG834050.08.815935120
GTAGGGA732200.08.663897120
TAGGGAA706450.08.58003120
AGTGTAG347050.08.533632120
CGTCGTG1050950.08.431193120
TGTAGGG815950.08.319438120
GTCGTGT978850.08.24212120
GATCTCG217900.08.132871120
AGCGTCG1224250.08.0816965120
GTGTAGA345950.07.9703693120
GCGTCGT1120700.07.9546967120
GAGCGTC1330300.07.893523120
AAGAGCG1554500.07.7520986120
TAGATCT270600.07.70337120
GTAGATC375950.07.6539297120
AGAGCGG86800.07.6129336120
Produced by FastQC (version 0.11.5)