FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102667667
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG245750.016.122713120
CGTCGTG3144050.012.017801120
CGTGTAG2361800.011.984675120
TCGTGTA2543350.011.915189120
AGCGTCG3872350.011.377582120
GTCGTGT2863750.011.278072120
GTGTAGG2397250.011.001086120
GCGTCGT3425750.010.887631120
GAGCGTC4385700.010.76583120
AAGAGCG5362850.010.594166120
AGAGCGT4758750.09.995038120
GTAGGGA2080750.09.904706120
TGTAGGG2422650.09.649237120
TCGGAAG7600650.09.503308120
ATCGGAA8047100.09.368473120
CGGAAGA7132000.09.306243120
TAGGGAA2015600.09.26581120
GATCGGA8647900.08.983486120
AGATCGG9403500.08.908342120
GAAGAGC6949900.08.67679120
Produced by FastQC (version 0.11.5)