FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29597520
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG511800.011.4004545120
TCGTGTA546350.011.198045120
AGCGTCG806000.011.038204120
CGTCGTG664400.010.505725120
GAGCGTC921950.010.441062120
GTCGTGT609400.010.217507120
GCGTCGT716200.010.217191120
AAGAGCG1120100.09.874757120
GTGTAGG540500.09.746818120
AGAGCGT996300.09.667935120
GATCGGA1759250.09.271443120
TCGGAAG1568850.09.236344120
GCGTCGG76200.09.175931120
GTAGGGA478700.09.154152120
CGGAAGA1490300.09.144794120
ATCGGAA1635900.08.868833120
AGATCGG1912000.08.827084120
TGTAGGG554250.08.42836120
TAGGGAA481350.08.152055120
CAGATCG968550.08.065477120
Produced by FastQC (version 0.11.5)