FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69583561
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA903750.013.181405120
ACGTCTG1015800.013.019252120
ACACGTC1236550.012.5838375120
CACGTCT1163050.011.986832120
CACACGT1458450.011.544234120
GCACACG1624650.011.252504120
TCGGAAG3347100.09.758326120
CGGAAGA3147150.09.739467120
AGCACAC2096100.09.600384120
ATCGGAA3528300.09.571094120
AGAGCAC2507800.09.464509120
GAGCACA2376350.09.443429120
GATCGGA3789300.09.332825120
AGATCGG4130200.09.28103120
GTCTGAA1230250.08.944314120
TAGATCG908950.08.70201120
GAAGAGC3231200.08.6142645120
TCTGAAC1160050.08.427011120
AAGATCG1442450.08.404714120
AAGAGCA3043050.08.401124120
Produced by FastQC (version 0.11.5)