FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69583561
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG952700.013.590479120
TCGTGTA1030600.012.919494120
CGTCGTG1280050.012.785944120
GTCGTGT1173950.012.63915120
AGCGTCG1596500.012.283799120
GAGCGTC1869850.011.650172120
GCGTCGT1398900.011.64802120
GTGTAGG1032150.011.412967120
GCGTCGG142350.011.290269120
AGAGCGT2046150.010.743451120
AAGAGCG2334450.010.638863120
GTAGGGA900650.010.225532120
TGTAGGG1065700.010.070868120
TCGGAAG3334450.09.820311120
CGGAAGA3154400.09.579382120
ATCGGAA3504400.09.550181120
AGATCGG4104450.09.267101120
GATCGGA3756400.09.260435120
TAGGGAA926000.08.639021120
GAAGAGC3237000.08.51302120
Produced by FastQC (version 0.11.5)