FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19019351
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA267300.011.706531120
CACGTCT337950.011.698677120
ACACGTC356500.011.427545120
ACGTCTG298200.011.219952120
CACACGT416100.09.978728120
GCACACG453900.09.5719595120
CGGAAGA825950.09.318384120
TCGGAAG870000.09.00566120
AGATCGG1066550.08.92584120
ATCGGAA920650.08.908921120
GATCGGA980500.08.8806505120
AGCACAC574050.08.784521120
GAGCACA640500.08.465049120
AGAGCAC668100.08.430595120
CAGATCG512050.08.355665120
AAGAGCA819250.08.101825120
GAAGAGC853500.08.023477120
AAGATCG241350.07.779132120
GAGATCG402650.07.6817336120
TAGATCG104050.07.4605603120
Produced by FastQC (version 0.11.5)