FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72576200
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC526250.011.080489120
GATCGGA1600700.010.830801120
ATCGGAA1498350.010.506347120
AGATCGG1791650.010.455704120
CGTCTGA411050.010.189241120
GCACACG746100.010.138309120
TCGGAAG1465450.010.089309120
CGGAAGA1415600.09.539148120
ACGTCTG463550.09.515567120
CACGTCT532550.09.491747120
TAGATCG331150.09.249544120
CACACGT688800.09.033471120
AAGATCG644150.08.688068120
CAGATCG701350.08.674483120
GAGATCG723750.07.8821926120
AGAGCAC1311900.07.7244697120
AGCACAC1144100.07.6844625120
GAGCACA1311350.07.1265626120
GAAGAGC1766350.07.103242120
AAGAGCA1742850.06.7190843120
Produced by FastQC (version 0.11.5)