FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72576200
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG630950.013.23074120
GCGTCGT543750.012.341791120
CGTCGTG505450.012.050495120
GTCGTGT476200.011.729005120
TCGTGTA416550.011.457995120
GAGCGTC784200.011.082633120
AGAGCGT857200.010.826918120
GATCGGA1600700.010.81388120
AAGAGCG1013350.010.803782120
ATCGGAA1496550.010.5932120
CGTGTAG411000.010.543706120
AGATCGG1787050.010.4878025120
TCGGAAG1465350.010.157466120
CGGAAGA1422350.09.381274120
TAGATCG304750.09.124328120
CAGATCG718650.08.844007120
AAGATCG669600.08.476154120
GTGTAGG559550.07.8628693120
GAGATCG754300.07.8355594120
GAAGAGC1797350.07.1292796120
Produced by FastQC (version 0.11.5)