FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42172294
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG574650.011.342115120
ACACGTC681700.011.298584120
CGTCTGA528350.011.25493120
CACACGT811050.011.05346120
CACGTCT650750.010.847312120
GCACACG911650.010.5658045120
ATCGGAA1939650.09.990912120
TCGGAAG1834500.09.852343120
CGGAAGA1725750.09.765926120
AGCACAC1162150.09.40589120
GATCGGA2080250.09.364782120
AGAGCAC1389150.09.336181120
AGATCGG2264950.09.110807120
GAGCACA1322900.08.821994120
CAGATCG925500.08.634094120
GAGATCG834500.08.466029120
GTCACGT156050.08.3611145120
AAGAGCA1708400.08.330612120
ACGTCCG80150.08.176948120
GAAGAGC1800600.08.1411295120
Produced by FastQC (version 0.11.5)