FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74684865
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG171300.021.012482120
CGTGTAG3208500.010.591035120
TCGTGTA3450600.010.377355120
GTGTAGG3130050.010.249827120
CGTCGTG4126550.010.170095120
TAGGGAA2555250.010.041588120
GTAGGGA2683250.09.985831120
GTCGTGT3797050.09.948004120
AGCGTCG4925450.09.788108120
TGTAGGG3003600.09.72304120
GAGCGTC5439150.09.380709120
GAGTGTA1185500.09.321548120
GCGTCGT4455100.09.233946120
AAGAGCG6481150.08.805262120
AGTGTAG1134650.08.71188120
AGAGCGT5875150.08.674331120
GTAGATC1034700.08.252538120
GCCGTAT185500.08.195671120
AGAGTGT1603650.08.120023120
GATCTCG635500.07.895485120
Produced by FastQC (version 0.11.5)