FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59647137
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG980750.012.753798120
CGTGTAG607400.012.165988120
CGTCGTG789200.011.936474120
GTCGTGT727150.011.814875120
TCGTGTA649150.011.661181120
GCGTCGT853500.011.3680525120
GAGCGTC1143900.010.908512120
AAGAGCG1440750.010.562425120
AGAGCGT1258000.010.554282120
ATCGGAA2162650.010.036881120
TCGGAAG2059450.09.868878120
GATCGGA2322650.09.539469120
AGATCGG2539100.09.504715120
GTGTAGG703300.09.464904120
CGGAAGA1959700.09.353404120
GCGTCGG107800.08.8612585120
CAGATCG1189450.08.707794120
GTAGGGA647850.08.40177120
TGTAGGG760250.08.084186120
TAGATCG274150.08.064486120
Produced by FastQC (version 0.11.5)