FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19531757
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA344050.012.377847120
ACGTCTG381450.012.35024120
ACACGTC460700.012.137339120
CACGTCT425650.011.762169120
CACACGT538650.011.590325120
GCACACG588700.011.1684885120
AGTCACG98300.010.984023120
GAGCACA823500.010.078985120
AGCACAC740050.09.960292120
TCGGAAG1210250.09.723981120
CGGAAGA1131550.09.6060095120
GTCACGT91250.09.452901120
AGAGCAC882450.09.364669120
ATCGGAA1286150.09.3471155120
AGATCGG1500000.09.228972120
GATCGGA1377800.08.9749120
GAAGAGC1109950.08.738654120
TAGATCG263850.08.641649120
GTCTGAA441450.08.417074120
AAGATCG496450.08.347245120
Produced by FastQC (version 0.11.5)